six well tissue culture plates  (CELLTREAT Scientific)

Journal: bioRxiv

Article Title: Efficient replication of influenza D virus in the human airway underscores zoonotic potential

doi: 10.64898/2026.02.07.704474

Figure Lengend Snippet: IDV was propagated on MDCK cells (multiplicity of infection [MOI] 0.001) until cytopathic effect (CPE) reached >85% (∼2-3 days). Infectious virus titers were quantified by plaque assay on MDCK cells. ( A ) Representative plaque phenotypes from the different IDV isolates, with the optimal date of readout noted below. ( B ) Quantification of virus titers for each isolate. Data show the mean +/-SEM from three independent plaque assay titrations on MDCK cells. Statistical significance was determined using two-tailed Mann-Whitney test for swine-compared to bovine-origin IDV isolates (ns, not significant).

Article Snippet: Diluted virus stocks were then exposed to MDCK cells (7.5×10 6 cells plated in 56.7-cm 2 dishes; CELLTREAT, #CT-229106) for 1 h at 37 °C with 5% CO2 with gentle rocking every 15 min.

Techniques: Infection, Virus, Plaque Assay, Two Tailed Test, MANN-WHITNEY